SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term,Ala600) antibody

Antigen: SUMO1 Activating Enzyme Subunit 2 (UBA2)

Synonyms: ARX, SAE2, FLJ13058, HRIHFB2115

Binding Site: C-Term,Ala600

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387914

Additional Information

Alternative name: SAE2 (UBA2)

Gene ID: 10054

UniProt: Q9UBT2

Immunogen: This SAE2 (UBA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 593~623 amino acids from the C-terminal region of human UBA2.

Isotype: Ig

Description: Other names: Ubiquitin-like 2 activating enzyme E1B, SUMO-1 activating enzyme subunit 2, Anthracycline-associated resistance ARX, HRIHFB2115

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SAE2 (UBA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 593~623 amino acids from the C-terminal region of human UBA2.

Molecular Weight: 71223 DA

Comments:

Background: Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43% sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

The anti-UBA2 Antibody (C-term A600)(ABIN387914) is used in Western blot to detect UBA2 in human placenta tissue lysate.

Western blot analysis of anti-UBA2 Antibody (C-term A600) (ABIN387914) in K562 cell line lysates (35ug/lane). UBA2(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).