|+1 404 474 4654|
|+1 888 205 9894 (TF)|
SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term,Ala600) antibody
|Synonyms||ARX, SAE2, FLJ13058, HRIHFB2115|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||SAE2 (UBA2)|
|Immunogen||This SAE2 (UBA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 593~623 amino acids from the C-terminal region of human UBA2.|
|Description||Other names: Ubiquitin-like 2 activating enzyme E1B, SUMO-1 activating enzyme subunit 2, Anthracycline-associated resistance ARX, HRIHFB2115|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This SAE2 (UBA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 593~623 amino acids from the C-terminal region of human UBA2.|
|Molecular Weight||71223 DA|
Background: Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43% sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).
Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).
Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).
|Hosts||Rabbit (26), Goat (3), Mouse (1)|
|Reactivities||Human (27), Mouse (Murine) (6), Rat (Rattus) (4), Dog (Canine) (1), Monkey (1)|
|Applications||Western Blotting (WB) (24), ELISA (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Immunohistochemistry (IHC) (6), Immunofluorescence (IF) (2), Dot Blot (DB) (1)|
|Epitopes||C-Term (4), N-Term (3), AA 160-210 (1), AA 286-303 (1), C-Term,Glu616 (1)|