Ubiquitin-Conjugating Enzyme E2M (ube2m) (N-Term) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2M (ube2m)

Synonyms: UBC12, hUbc12, UBC-RS2, Ubc-rs2, MGC25453, MGC37197, 2510040H03Rik, ube2m, MGC75750, MGC80512, UBE2M, MGC151985

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387917

Additional Information

Alternative name: UBE2M

Gene ID: 9040

UniProt: NP_003960

Immunogen: This UBE2M antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7~37 amino acids from the N-terminal region of human UBE2M.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This UBE2M antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7~37 amino acids from the N-terminal region of human UBE2M.

Molecular Weight: 20900 DA

Comments:

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blot~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-UBE2M Antibody (N-term)(ABIN387917) in A2058 cell line lysates (35ug/lane). UBE2M(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Osaka, Kawasaki, Aida et al.: "A new NEDD8-ligating system for cullin-4A." in: Genes & development, Vol. 12, Issue 15, pp. 2263-8, 1998 (PubMed).

Gong, Yeh: "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway." in: The Journal of biological chemistry, Vol. 274, Issue 17, pp. 12036-42, 1999 (PubMed).