|+1 404 474 4654|
|+1 888 205 9894 (TF)|
SET Domain, Bifurcated 1 (SETDB1) (N-Term) antibody
|Synonyms||ESET, KG1T, KMT1E, KIAA0067, H3-K9-HMTase4, AU022152, MGC90670, mKIAA0067, SETDB1, NV10069, fc07d03, wu:fc07d03, si:ch211-81a5.6|
Alternatives Western Blotting (WB), ELISA
|4 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This SETDB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130~160 amino acids from the N-terminal region of human SETDB1.|
|Description||Other names: Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, SET domain bifurcated 1, ERG-associated protein with SET domain, ESET,, KMT1E|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This SETDB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130~160 amino acids from the N-terminal region of human SETDB1.|
|Molecular Weight||143157 DA|
Background: The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Signaling, Protein Modifications, Cancer, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
|Western blot analysis of SETDB1 (arrow) using rabbit polyclonal SETDB1 Antibody (N-term) (ABIN387923). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SETDB1 gene (Lane 2) (Origene Technologies).|
Schultz, Ayyanathan, Negorev et al.: "SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins." in: Genes & development, Vol. 16, Issue 8, pp. 919-32, 2002 (PubMed).
Wang, An, Cao et al.: "mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression." in: Molecular cell, Vol. 12, Issue 2, pp. 475-87, 2003 (PubMed).
Sarraf, Stancheva: "Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly." in: Molecular cell, Vol. 15, Issue 4, pp. 595-605, 2004 (PubMed).
Ichimura, Watanabe, Sakamoto et al.: "Transcriptional repression and heterochromatin formation by MBD1 and MCAF/AM family proteins." in: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 13928-35, 2005 (PubMed).
|Hosts||Rabbit (32), Mouse (13)|
|Reactivities||Human (44), Mouse (Murine) (29), Rat (Rattus) (20), Cow (Bovine) (18), Dog (Canine) (18), Horse (Equine) (18), Pig (Porcine) (18), Rabbit (18), Sheep (Ovine) (18), Monkey (9)|
|Applications||ELISA (29), Western Blotting (WB) (28), Immunofluorescence (IF) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (IHC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)|
|Epitopes||C-Term (3), AA 1058-1075 (2), AA 475-489 (1), N-Term (1)|