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Histone Deacetylase 4 (HDAC4) (AA 1052-1084), (C-Term) antibody

Details for Product No. ABIN387951, Supplier: Login to see
Antigen
  • CG1770
  • DHDAC4
  • Dmel\\CG1770
  • GC1770
  • HDAC
  • HDAC4a
  • dHDAC4
  • dmHDA405
  • hdac4
  • dana_GLEANR_19966
  • DanaGF18710
  • GF18710
  • HDAC4
  • AHO3
  • BDMR
  • HA6116
  • HD4
  • HDAC-4
  • HDAC-A
  • HDACA
  • 4932408F19Rik
  • AI047285
  • wu:fa96d08
  • wu:fc56f08
  • zgc:152701
Epitope
AA 1052-1084, C-Term
46
34
20
15
10
10
6
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
259
101
71
8
3
3
2
2
1
1
1
1
1
Host
Rabbit
226
39
2
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
10
9
9
4
4
4
3
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
235
85
50
32
30
22
13
12
11
5
3
1
1
1
1
Supplier
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Immunogen This HDAC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1052-1084 AA from the C-terminal region of human HDAC4.
Clone RB5648
Isotype Ig
Specificity This HDAC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human HDAC4.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name HDAC4 (HDAC4 Antibody Abstract)
Background DNA is wrapped around histone proteins to form nucleosomes and chromatin fiber, a higher-order structure. Chromatin can become alternatively revealed to or concealed from transcription factors. Acetylation of lysine residues induces conformational changes in core histones by destabilizing nucleosomes and allowing transcription factors access to recognition elements in DNA. Deacetylation of histones by histone deacetylases (HDACs) reseals the chromosomal package, leading to a repression of transcription. HDAC4 does not bind DNA directly, but rather through MEF2C and MEF2D. Binding of the N terminus of HDAC4 to MEF2C represses MEF2C transcription activity. The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2. Experimental conditions leading to the suppression of HDAC4 binding to NCOR2 and to HDAC3 result in loss of enzymatic activity associated with HDAC4, indicating regulation of transcription by bridging the enzymatically active NCOR2-HDAC3 complex and select transcription factors. HDAC4 and MITR contain calmodulin-binding domains that overlap with their MEF2 binding domains. Binding of calmodulin to HDAC4 leads to its dissociation from MEF2, relieving MEF2 from the transcriptional repression by HDAC4. Together, HDAC4, MITR, and CABIN1 constitute a family of calcium-sensitive transcriptional repressors of MEF2. In murine studies, HDAC4, which is expressed in prehypertrophic chondrocytes, interacts with and inhibits the activity of Runx2 in mice, a transcription factor necessary for chondrocyte hypertrophy, establishing HDAC4 as a central regulator of chondrocyte hypertrophy and skeletogenesis.
Synonyms: Histone deacetylase 4, HD4, KIAA0288
Molecular Weight 119069 DA
Gene ID 9759
UniProt P56524
Research Area Signaling, Protein Modifications, Transcription Factors, Chromatin, Neurology, Cell Structure
Pathways
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Histone Deacetylase 4 (HDAC4) (AA 1052-1084), (C-Term) antibody (ABIN387951) The anti-HDAC4 Pab (ABIN387951) is used in Western blot to detect HDAC4 in mouse brai...
Background publications Kao, McKenna, Guenther et al.: "Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response." in: The Journal of cell biology, Vol. 160, Issue 7, pp. 1017-27, 2003 (PubMed).

Youn, Grozinger, Liu: "Calcium regulates transcriptional repression of myocyte enhancer factor 2 by histone deacetylase 4." in: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 22563-7, 2000 (PubMed).