Histone Deacetylase 5 (HDAC5) (N-Term) antibody

Antigen: Histone Deacetylase 5 (HDAC5)

Synonyms: HD5, NY-CO-9, FLJ90614, Hdac4, mHDA1, AI426555, mKIAA0600, Hd6, Sfc6, Hdac5, mHDA2, HDAC5, MGC134463, HDA5, HISTONE DEACETYLASE 5, MAF19.7, MAF19_7, DKFZp459O115

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387953

Additional Information

Alternative name: HDAC5

Gene ID: 10014

UniProt: Q9UQL6

Immunogen: This HDAC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human HDAC5.

Isotype: Ig

Description: Other names: Histone deacetylase 5, HD5, Antigen NY-CO-9, KIAA0600

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HDAC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human HDAC5.

Molecular Weight: 121991 DA

Comments:

Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with the HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This protein is thought to be associated with colon cancer.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Flow cytometry

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Cell Cycle, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

The anti-HDAC5 Pab (ABIN387953) is used in Western blot to detect HDAC5 in 293 cell lysate.

Publications

Product: Grozinger, Schreiber: "Regulation of histone deacetylase 4 and 5 and transcriptional activity by 14-3-3-dependent cellular localization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 14, pp. 7835-40, 2000 (PubMed).

Huang, Tan, Gosink et al.: "Histone deacetylase 5 is not a p53 target gene, but its overexpression inhibits tumor cell growth and induces apoptosis." in: Cancer research, Vol. 62, Issue 10, pp. 2913-22, 2002 (PubMed).

Berger, Bieniossek, Schaffitzel et al.: "Direct interaction of Ca2+/calmodulin inhibits histone deacetylase 5 repressor core binding to myocyte enhancer factor 2." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17625-35, 2003 (PubMed).