SET Domain Containing 8 Pseudogene 1 (SETD8P1) (N-Term) antibody

Antigen: SET Domain Containing 8 Pseudogene 1 (SETD8P1)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387996

Additional Information

Alternative name: SET7

Gene ID: 80854

UniProt: Q8WTS6

Immunogen: This SET7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27~57 amino acids from the N-terminal region of human SET7.

Isotype: Ig

Description: Other names: Histone H3-K4 methyltransferase, H3-K4-HMTase, SET domain-containing protein 7, Set9, SET7/9,, KMT7

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SET7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27~57 amino acids from the N-terminal region of human SET7.

Molecular Weight: 40721 DA

Comments:

Background: Histone methyltransferases (HMTases) selectively methylate evolutionarily conserved arginine or lysine residues, primarily in the N-terminal tails of histones H3 and H4. Signal transduction pathways affecting the N-terminal tails of histones lead to a number of post-translational modifications including acetylation, phosphorylation, poly(ADP-ribosylation), ubiquitination and methylation. These modifications play critical roles in regulating chromatin structure and gene expression. Set7/9 is a histone specific HMTase that methylates histone H3 lysine 4. Set7/9 transfers methyl groups to lysine 4 of histone H3 in complex with S-adenosyl-L-methionine. In yeast, H4-K20 methylation does not have any apparent role in the regulation of gene expression or heterochromatin function, rather it appears to play a role in DNA damage response. Loss of Set9 activity or mutation of H4-K20 markedly impairs yeast cell survival after genotoxic challenge and compromises the ability of cells to maintain checkpoint mediated cell cycle arrest. Genetic experiments link Set9 to Crb2, a homolog of the mammalian checkpoint protein 53BP1, and the enzyme is required for Crb2 localization to sites of DNA damage.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-SET7 Pab(ABIN387996) in HL60 cell line lysate (35ug/lane). SET7(arrow) was detected using the purified Pab (1:60 dilution).

Publications

Product: Nishioka, Chuikov, Sarma et al.: "Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation." in: Genes & development, Vol. 16, Issue 4, pp. 479-89, 2002 (PubMed).

Kwon, Chang, Kwak et al.: "Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet." in: The EMBO journal, Vol. 22, Issue 2, pp. 292-303, 2003 (PubMed).

Xiao, Jing, Wilson et al.: "Structure and catalytic mechanism of the human histone methyltransferase SET7/9." in: Nature, Vol. 421, Issue 6923, pp. 652-6, 2003 (PubMed).

Wysocka, Myers, Laherty et al.: "Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1." in: Genes & development, Vol. 17, Issue 7, pp. 896-911, 2003 (PubMed).

Chuikov, Kurash, Wilson et al.: "Regulation of p53 activity through lysine methylation." in: Nature, Vol. 432, Issue 7015, pp. 353-60, 2004 (PubMed).