|+1 404 474 4654|
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SUMO1 Activating Enzyme Subunit 1 (SAE1) (C-Term) antibody
|Synonyms||AOS1, SUA1, UBLE1A, FLJ3091, HSPC140|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||400 µL (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||SAE1 (AOS1)|
|Immunogen||This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272~302 amino acids from the C-terminal region of human SAE1.|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272~302 amino acids from the C-terminal region of human SAE1.|
|Molecular Weight||38450 DA|
Background: Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respects to size and ubiquity ?to which eukaryotic proteins are subject. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied for over two decades. Recently a new group of ubiquitin-like (Ubl) proteins have come to light. One of the most intriguing of them is SUMO (small ubiquitin-like modifier, ~12kDa) also known as Sentrin. SUMO family has been described in vertebrates: SUMO-1 and the closest homologs SUMO-2 and SUMO-3. SUMO have been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, IkB-alpha, Androgen receptor (AR), GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast Septins, and viral CMV-IE1/2, EBV-BZLF1, HPV/BPV-E1. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes. SUMO research enters now an exciting phase with a promise to help understanding how cells orchestrate the complexities of rapidly regulating protein level and activity.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western Blot~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Protein Modifications, Cancer, Transcription Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).
Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).
Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).
|Hosts||Rabbit (49), Mouse (5), Goat (4), Sheep (1)|
|Reactivities||Human (51), Rat (Rattus) (11), Mouse (Murine) (10), Cow (Bovine) (9), Dog (Canine) (5)|
|Applications||Western Blotting (WB) (54), ELISA (45), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunofluorescence (IF) (7), Immunohistochemistry (IHC) (7), Fluorescence Microscopy (FM) (3), Immunocytochemistry (ICC) (2), Dot Blot (Dot) (1)|
|Epitopes||C-Term (12), N-Term (8), pSer185 (5), Internal Region (4)|