DIM1 Dimethyladenosine Transferase 1 Homolog (S. Cerevisiae) (DIMT1) (C-Term) antibody

Antigen: DIM1 Dimethyladenosine Transferase 1 Homolog (S. Cerevisiae) (DIMT1)

Synonyms: DIM1, DIMT1, HUSSY5, HSA9761, Dimt1l, AV111347, 1500031M22Rik, Dimt1, RGD1311752, wu:fc96g04, zgc:101122, MGC137746

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388013

Additional Information

Alternative name: DIMT1L

Gene ID: 27292

UniProt: Q9UNQ2

Immunogen: This DIMT1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 278~308 amino acids from the C-terminal region of human DIMT1L.

Isotype: Ig

Description: Other names: Probable dimethyladenosine transferase, S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase, 18S rRNA dimethylase, DIM1 dimethyladenosine transferase 1-like, HUSSY-05DIMT1, DIMT1L

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DIMT1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 278~308 amino acids from the C-terminal region of human DIMT1L.

Molecular Weight: 35236 DA

Comments:

Background: DIMT1L specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications

Restrictions: For Research Use only

Images

Western blot analysis of DIMT1L antibody (C-term)(ABIN388013) in Ramos cell line lysates (35ug/lane). DIMT1L (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue with DIMT1L Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

DIMT1L Antibody (C-term) (ABIN388013) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Law, Zhang, Klein-Szanto et al.: "Cell cycle-regulated processing of HEF1 to multiple protein forms differentially targeted to multiple subcellular compartments." in: Molecular and cellular biology, Vol. 18, Issue 6, pp. 3540-51, 1998 (PubMed).