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Lysine (K)-Specific Demethylase 1A (KDM1A) (AA 108-142), (N-Term) antibody

Details for Product No. ABIN388021, Supplier: Log in to see
Antigen
  • KDM1
  • AOF2
  • KDM1A
  • DKFZp459N2450
  • aof2
  • kdm1
  • lsd1
  • xLSD1
  • BHC110
  • LSD1
  • 1810043O07Rik
  • AA408884
  • Aof2
  • D4Ertd478e
  • Kdm1
  • Lsd1
  • mKIAA0601
  • RGD1562975
  • wu:fb82e06
  • wu:fc51a02
  • wu:fj36a05
  • wu:fj66b06
  • ARABIDOPSIS LYSINE-SPECIFIC HISTONE DEMETHYLASE
  • ATLSD1
  • ATSWP1
  • F23N19.21
  • F23N19_21
  • LSD1-like 1
  • LYSINE-SPECIFIC HISTONE DEMETHYLASE
  • SWP1
Epitope
AA 108-142, N-Term
23
10
8
8
8
7
5
4
3
3
3
3
3
3
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
177
80
70
13
9
2
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
143
38
1
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
152
51
51
38
27
22
12
6
6
6
3
2
1
1
1
Supplier
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Immunogen This LSD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-142 AA from the N-terminal region of human LSD1.
Clone RB7579-RB7580
Isotype Ig
Specificity This LSD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-term of human LSD1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name LSD1 (KDM1A Antibody Abstract)
Background LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
Synonyms: Lysine-specific histone demethylase 1, Amine oxidase flavin containing domain protein 2, BRAF35-HDAC complex protein BHC110, AOF2, KIAA0601, KDM1
Molecular Weight 92903 DA
Gene ID 23028
UniProt O60341
Research Area Signaling, Protein Modifications, Cancer, Transcription Factors, Chromatin, Neurology, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Lysine (K)-Specific Demethylase 1A (KDM1A) (AA 108-142), (N-Term) antibody (ABIN388021) Western blot analysis of anti-LSD1 Pab (ABIN388021) in mouse brain tissue lysate (35 ...
Western Blotting (WB) image for anti-Lysine (K)-Specific Demethylase 1A (KDM1A) (AA 108-142), (N-Term) antibody (ABIN388021) Western blot analysis of AOF2 (arrow) using LSD1 Antibody (N-term) (ABIN388021).293 c...
Immunohistochemistry (IHC) image for anti-Lysine (K)-Specific Demethylase 1A (KDM1A) (AA 108-142), (N-Term) antibody (ABIN388021) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications Forneris, Binda, Vanoni et al.: "Histone demethylation catalysed by LSD1 is a flavin-dependent oxidative process." in: FEBS letters, Vol. 579, Issue 10, pp. 2203-7, 2005 (PubMed).

Shi, Lan, Matson et al.: "Histone demethylation mediated by the nuclear amine oxidase homolog LSD1." in: Cell, Vol. 119, Issue 7, pp. 941-53, 2004 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Hakimi, Dong, Lane et al.: "A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7234-9, 2003 (PubMed).

Hakimi, Bochar, Chenoweth et al.: "A core-BRAF35 complex containing histone deacetylase mediates repression of neuronal-specific genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 11, pp. 7420-5, 2002 (PubMed).

Humphrey, Wang, Russanova et al.: "Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1." in: The Journal of biological chemistry, Vol. 276, Issue 9, pp. 6817-24, 2001 (PubMed).