Lysine (K)-Specific Demethylase 1A (KDM1A) (AA 457-490), (Center) antibody

Details for Product No. ABIN388022
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Antigen
Synonyms
AOF2, BHC110, KDM1, LSD1, 1810043O07Rik, AA408884, Aof2, D4Ertd478e, Kdm1, Lsd1, mKIAA0601, RGD1562975, aof2, kdm1, wu:fb82e06, wu:fc51a02, wu:fj36a05, wu:fj66b06, ARABIDOPSIS LYSINE-SPECIFIC HISTONE  ... show more
AOF2, BHC110, KDM1, LSD1, 1810043O07Rik, AA408884, Aof2, D4Ertd478e, Kdm1, Lsd1, mKIAA0601, RGD1562975, aof2, kdm1, wu:fb82e06, wu:fc51a02, wu:fj36a05, wu:fj66b06, ARABIDOPSIS LYSINE-SPECIFIC HISTONE DEMETHYLASE, ATLSD1, ATSWP1, F23N19.21, F23N19_21, LSD1-like 1, LYSINE-SPECIFIC HISTONE DEMETHYLASE, SWP1, KDM1A, DKFZp459N2450, lsd1, xLSD1 show less
Epitope
AA 457-490, Center
(16), (6), (6), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Reactivity
Human
(134), (70), (60), (33), (26), (26), (24), (14), (3), (2), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(105), (32), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(115), (55), (41), (27), (20), (16), (7), (4), (4), (4), (3), (1), (1)
Pubmed 6 references available
Catalog no. ABIN388022
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This LSD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-490 AA from the Central region of human LSD1.
Clone RB7581
Isotype Ig
Specificity This LSD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected in the center region of human LSD1.
Predicted Reactivity Mouse (Murine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name LSD1
Background LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
Synonyms: Lysine-specific histone demethylase 1, Amine oxidase flavin containing domain protein 2, BRAF35-HDAC complex protein BHC110, AOF2, KIAA0601, KDM1
Molecular Weight 92903 DA
Gene ID 23028
UniProt O60341
Research Area Signaling, Protein Modifications, Cancer, Transcription Factors, Chromatin, Neurology, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Lysine (K)-Specific Demethylase 1A (KDM1A) (AA 457-490), (Center) antibody Western blot analysis of AOF2 (arrow) using LSD1 Antibody (Center) (ABIN388022). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2) (Origene Technologies).
anti-Lysine (K)-Specific Demethylase 1A (KDM1A) (AA 457-490), (Center) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Humphrey, Wang, Russanova et al.: "Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1." in: The Journal of biological chemistry, Vol. 276, Issue 9, pp. 6817-24, 2001 (PubMed).

Hakimi, Bochar, Chenoweth et al.: "A core-BRAF35 complex containing histone deacetylase mediates repression of neuronal-specific genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 11, pp. 7420-5, 2002 (PubMed).

Hakimi, Dong, Lane et al.: "A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7234-9, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Shi, Lan, Matson et al.: "Histone demethylation mediated by the nuclear amine oxidase homolog LSD1." in: Cell, Vol. 119, Issue 7, pp. 941-53, 2004 (PubMed).

Forneris, Binda, Vanoni et al.: "Histone demethylation catalysed by LSD1 is a flavin-dependent oxidative process." in: FEBS letters, Vol. 579, Issue 10, pp. 2203-7, 2005 (PubMed).

Hosts (105), (32), (1)
Reactivities (134), (70), (60), (33), (26), (26), (24), (14), (3), (2), (1), (1), (1), (1), (1), (1)
Applications (115), (55), (41), (27), (20), (16), (7), (4), (4), (4), (3), (1), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (16), (6), (6), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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