SMT3 Suppressor of Mif Two 3 Homolog 3 (S. Cerevisiae) (SUMO3) (C-Term,Pro94) antibody

Antigen: SMT3 Suppressor of Mif Two 3 Homolog 3 (S. Cerevisiae) (SUMO3)

Synonyms: SMT3A, SMT3H1, SUMO-3, Smt3h1, AA409334, AW121497, AW536077, D10Ertd345e, 2810014B19Rik, sumo3, sumo2, fa94a11, zgc:65847, zgc:85643, im:7141764, wu:fa94a11, wu:fb55d09, fc66f10, fl11h12, zgc:86902, wu:fc66f10, wu:fl11h12, SUMO3, ATSUMO3, MCO15.12, MCO15_12, SMALL UBIQUITIN-LIKE MODIFIER 3, SUM3, SUMO 3

Binding Site: C-Term,Pro94

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388036

Additional Information

Alternative name: SUMO3

Gene ID: 6612

UniProt: P55854

Immunogen: This SUMO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74~103 amino acids from the C-terminal region of human SUMO3.

Isotype: Ig

Description: Other names: Small ubiquitin-related modifier 3, SUMO-3, Ubiquitin-like protein SMT3A, SMT3 homolog 1, SUMO-2, SMT3A, SMT3H1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SUMO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74~103 amino acids from the C-terminal region of human SUMO3.

Molecular Weight: 11637 DA

Comments:

Background: Covalent modification of target lysines by SUMO (small ubiquitin-like modifier) modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction. SUMO does not seem to be involved in protein degradation and may in fact function as an antagonist of ubiquitin in the degradation process. The SUMO family consists of SUMO1 and closely related homologs SUMO2, SUMO3, and SUMO4. Sumoylation has been shown to regulate a wide range of proteins, including MDM2, PIAS, PML, RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, PARK2, WRN, Sp100, IkB-alpha, Androgen receptor (AR), GLUT1/4, CaMK, DNMT3B, TDG, HIF1A, CHD3, EXOSC9, RAD51, and viral targets such as CMV-IE1/2, EBV-BZLF1, and HPV/BPV-E1.

Synonyms: SMT3A, SMT3H1, SUMO-3, Smt3h1, AA409334, AW121497, AW536077, D10Ertd345e, 2810014B19Rik, sumo3, sumo2, fa94a11, zgc:65847, zgc:85643, im:7141764, wu:fa94a11, wu:fb55d09, fc66f10, fl11h12, zgc:86902, wu:fc66f10, wu:fl11h12, SUMO3, ATSUMO3, MCO15.12, MCO15_12, SMALL UBIQUITIN-LIKE MODIFIER 3, SUM3, SUMO 3

Application Details

Application Notes: Suggested Dilution: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

The anti-SUMO3 polyclonal antibody (ABIN388036) is used in Western blot to detect SUMO3 in Jurkat cell lysate.

Publications

Product: Lapenta, Chiurazzi, van der Spek et al.: "SMT3A, a human homologue of the S. cerevisiae SMT3 gene, maps to chromosome 21qter and defines a novel gene family." in: Genomics, Vol. 40, Issue 2, pp. 362-6, 1997 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).