SUMO1 Activating Enzyme Subunit 1 (SAE1) antibody

Antigen: SUMO1 Activating Enzyme Subunit 1 (SAE1)

Synonyms: AOS1, SUA1, UBLE1A, FLJ3091, HSPC140

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388076

Additional Information

Alternative name: AOS1

Gene ID: 10055

UniProt: Q9UBE0

Immunogen: This AOS1 antibody is generated from rabbits immunized with a recombinant protein encoding full length of human AOS1.

Isotype: Ig

Description: Other names: SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, SAE1, AOS1, SUA1, UBLE1A

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AOS1 antibody is generated from rabbits immunized with a recombinant protein encoding full length of human AOS1.

Molecular Weight: 38450 DA

Comments:

Background: The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

The AOS1 Antibody (ABIN388076) is used in Western blot to detect AOS1 in mouse colon tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).