Pan SUMO antibody

Antigen: Pan SUMO

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388091

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Gene ID: 6612

Swiss-Prot: P55854

Immunogen: "This Pan SUMO antibody recoganize all 3 SUMO isoforms, including human SUMO1, SUMO2 and SUMO3. This antibody is generated from rabbits immunized with a recombinant protein encoding full length of human SUMO1."

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Small ubiquitin-related modifier 1, SUMO-1, Ubiquitin-like protein SMT3C, SMT3 homolog 3, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein UBL1, GAP modifying protein 1, GMP1, Sentrin, SMT3C, SMT3H3, UBL1, Small ubiquitin-related modifier 2,

Specificity: This antibody recoganize all 3 SUMO isoforms, including human SUMO1, SUMO2 and SUMO3. This antibody is generated from rabbits immunized with a recombinant protein encoding full length of human SUMO1.

Molecular Weight: 11557 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

The Pan SUMO Antibody (ABIN388091) is used in Western blot to detect SUMO1 in GST(26kd)-Sumo1 protein bacterial lysate.