EVX2 (C-Term) antibody

Antigen: EVX2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388194

Additional Information

Gene ID: 14029

UniProt: P49749

Immunogen: This Evx2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 446~475 amino acids from the C-terminal region of mouse EVX2.

Isotype: Ig

Description: Other names: Homeobox even-skipped homolog protein 2, EVX-2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Evx2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 446~475 amino acids from the C-terminal region of mouse EVX2.

Molecular Weight: 47902 DA

Comments:

Background: Evx-2, a protein related to the Drosophila even-skipped (eve) , is expressed in limbs like the neighbouring Hoxd genes. Evx-2 functions like a Hoxd gene.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of anti-Evx2 Pab (ABIN388194) in mouse kidney tissue lysates (35ug/lane). Evx2(arrow) was detected using the purified Pab (1:60 dilution).

Publications

Product: Tarchini, Huynh, Cox et al.: "HoxD cluster scanning deletions identify multiple defects leading to paralysis in the mouse mutant Ironside." in: Genes & development, Vol. 19, Issue 23, pp. 2862-76, 2005 (PubMed).

Tarchini, Duboule: "Control of Hoxd genes' collinearity during early limb development." in: Developmental cell, Vol. 10, Issue 1, pp. 93-103, 2006 (PubMed).

Montavon, Le Garrec, Kerszberg et al.: "Modeling Hox gene regulation in digits: reverse collinearity and the molecular origin of thumbness." in: Genes & development, Vol. 22, Issue 3, pp. 346-59, 2008 (PubMed).