Retina and Anterior Neural Fold Homeobox (RAX) (C-Term) antibody

Antigen: Retina and Anterior Neural Fold Homeobox (RAX)

Synonyms: RAX, rx, rx1, Rx2A, Xrax, Xrx1, rax

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388196

Additional Information

Alternative name: Rax

Gene ID: 30062

UniProt: Q9Y2V3

Immunogen: This Rax antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317~346 amino acids from the C-terminal region of human RAX.

Isotype: Ig

Description: Other names: Retinal homeobox protein Rx, Retina and anterior neural fold homeobox protein, RAX

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Rax antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317~346 amino acids from the C-terminal region of human RAX.

Molecular Weight: 36805 DA

Comments:

Background: Rax plays a critical role in eye formation by regulating the initial specification of retinal cells and/or their subsequent proliferation. It binds to the photoreceptor conserved element-I (PCE-1/Ret 1) in the photoreceptor cell-specific arrestin promoter.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-Rax Pab (ABIN388196) in Y79 cell line lysates (35ug/lane). Rax(arrow) was detected using the purified Pab (1:60 dilution).

Publications

Product: Mikkola, Bruun, Holm et al.: "Superactivation of Pax6-mediated transactivation from paired domain-binding sites by dna-independent recruitment of different homeodomain proteins." in: The Journal of biological chemistry, Vol. 276, Issue 6, pp. 4109-18, 2001 (PubMed).

Voronina, Kozhemyakina, OKernick et al.: "Mutations in the human RAX homeobox gene in a patient with anophthalmia and sclerocornea." in: Human molecular genetics, Vol. 13, Issue 3, pp. 315-22, 2004 (PubMed).