alpha-Fetoprotein (AFP) (AA 300-329), (Center) antibody

Details for Product No. ABIN388199
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Antigen
Synonyms FETA, HPAFP
Epitope
AA 300-329, Center
(25), (11), (7), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(500), (40), (35), (10), (9), (3), (3), (2), (1), (1)
Host
Rabbit
(295), (206), (31), (6), (5), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(21), (16), (14), (5), (5), (5), (5), (5), (5), (5), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(255), (239), (132), (68), (53), (50), (36), (36), (31), (24), (20), (7), (6), (6), (6), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN388199
291.50 $
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Immunogen This AFP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-329 AA from the Central region of human AFP.
Clone RB13358
Isotype Ig
Specificity This AFP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307~337 amino acids from the Center region of human AFP.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name AFP (alpha 1 Fetoprotein)
Background Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Synonyms: Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, AFP, HPAFP
Molecular Weight 68678 DA
Gene ID 174
UniProt P02771
Research Area Stem Cells, Transcription Factors, Signaling, Chromatin
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-alpha-Fetoprotein (AFP) (AA 300-329), (Center) antibody Western blot analysis of AFP (arrow) using rabbit polyclonal AFP Antibody (Center) (ABIN388199). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the AFP gene (Lane 2) (Origene Technologies).
anti-alpha-Fetoprotein (AFP) (AA 300-329), (Center) antibody (2) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AFP antibody (Center) (ABIN388199), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Cajaiba, Sarita-Reyes, Zambrano et al.: "Mesenchymal hamartoma of the liver associated with features of Beckwith-Wiedemann syndrome and high serum alpha-fetoprotein levels." in: Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society, Vol. 10, Issue 3, pp. 233-8, 2007 (PubMed).

Yamashita, Forgues, Wang et al.: "EpCAM and alpha-fetoprotein expression defines novel prognostic subtypes of hepatocellular carcinoma." in: Cancer research, Vol. 68, Issue 5, pp. 1451-61, 2008 (PubMed).

Validation Images
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