Cadherin 9 (CDH9) (C-Term) antibody

Antigen: Cadherin 9 (CDH9)

Synonyms: MGC125386, MGC157284

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388203

Additional Information

Alternative name: CDH9

Gene ID: 1007

UniProt: Q9ULB4

Immunogen: This CDH9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 708~737 amino acids from the C-terminal region of human CDH9.

Isotype: Ig

Description: Other names: Cadherin-9

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CDH9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 708~737 amino acids from the C-terminal region of human CDH9.

Molecular Weight: 88689 DA

Comments:

Background: CDH9 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CDH9 (arrow) using rabbit polyclonal CDH9 Antibody (C-term) (ABIN388203). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH9 gene (Lane 2) (Origene Technologies).

Flow cytometric analysis of MCF-7 cells using CDH9 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Nollet, Kools, van Roy: "Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members." in: Journal of molecular biology, Vol. 299, Issue 3, pp. 551-72, 2000 (PubMed).

Shimoyama, Tsujimoto, Kitajima et al.: "Identification of three human type-II classic cadherins and frequent heterophilic interactions between different subclasses of type-II classic cadherins." in: The Biochemical journal, Vol. 349, Issue Pt 1, pp. 159-67, 2001 (PubMed).