Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) (C-Term) antibody

Antigen: Sprouty-Related, EVH1 Domain Containing 1 (SPRED1)

Synonyms: AL024345, AW047647, 5730461F13Rik, NFLS, FLJ33903, fb14c08, fj51c03, zgc:85707, wu:fb14c08, wu:fj51c03, Xspred1, MGC79569, Xtspred1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388229

Additional Information

Alternative name: SPRED1

Gene ID: 161742

UniProt: Q7Z699

Immunogen: This SPRED1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309~339 amino acids from the C-terminal region of human SPRED1.

Isotype: Ig

Description: Other names: Sprouty-related, EVH1 domain-containing protein 1, Spred-1, SPRED1,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SPRED1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309~339 amino acids from the C-terminal region of human SPRED1.

Molecular Weight: 50477 DA

Comments:

Background: SPRED1 is a tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of SPRED1 Antibody (C-term) polyclonal antibody(ABIN388229)(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPRED1 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human brain tissue reacted with SPRED1 antibody (C-term) (ABIN388229), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Yoshida, Hisamoto, Akiba et al.: "Spreds, inhibitors of the Ras/ERK signal transduction, are dysregulated in human hepatocellular carcinoma and linked to the malignant phenotype of tumors." in: Oncogene, Vol. 25, Issue 45, pp. 6056-66, 2006 (PubMed).

Brems, Chmara, Sahbatou et al.: "Germline loss-of-function mutations in SPRED1 cause a neurofibromatosis 1-like phenotype." in: Nature genetics, Vol. 39, Issue 9, pp. 1120-6, 2007 (PubMed).