Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) (N-Term), (AA 44-73) antibody

Details for Product No. ABIN388231
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Antigen
Epitope
N-Term, AA 44-73
(8), (8), (6), (6), (5), (4), (4), (4), (1)
Reactivity
Human
(54), (16), (16)
Host
Rabbit
(46), (8)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (4), (4), (4)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(54), (43), (25), (11), (7), (1), (1), (1)
Pubmed 2 references available
Quantity 400 μL
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Catalog No. ABIN388231
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Immunogen This SPRED1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-73 AA from the N-terminal region of human SPRED1.
Clone RB13254
Isotype Ig
Specificity This SPRED1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51~81 amino acids from the N-terminal region of human SPRED1.
Predicted Reactivity Mouse (Murine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SPRED1
Background SPRED1 is a tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.
Synonyms: Sprouty-related, EVH1 domain-containing protein 1, Spred-1, SPRED1,
Molecular Weight 50477 DA
Gene ID 161742
UniProt Q7Z699
Research Area Stem Cells, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) (N-Term), (AA 44-73) antibody Western blot analysis of SPRED1 (arrow) using rabbit polyclonal SPRED1 Antibody (N-term) (ABIN388231). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the SPRED1 gene (Lane 2) (Origene Technologies).
anti-Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) (N-Term), (AA 44-73) antibody (2) Formalin-fixed and paraffin-embedded human brain tissue reacted with SPRED1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Yoshida, Hisamoto, Akiba et al.: "Spreds, inhibitors of the Ras/ERK signal transduction, are dysregulated in human hepatocellular carcinoma and linked to the malignant phenotype of tumors." in: Oncogene, Vol. 25, Issue 45, pp. 6056-66, 2006 (PubMed).

Brems, Chmara, Sahbatou et al.: "Germline loss-of-function mutations in SPRED1 cause a neurofibromatosis 1-like phenotype." in: Nature genetics, Vol. 39, Issue 9, pp. 1120-6, 2007 (PubMed).

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