Insulin-Degrading Enzyme (IDE) (AA 406-435), (Center) antibody

Details for Product No. ABIN388241
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Antigen
Synonyms zgc:162603, IDE, 1300012G03Rik, 4833415K22Rik, AA675336, AI507533, INSDEGM, INSULYSIN
Epitope
AA 406-435, Center
(17), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(66), (36), (26), (25), (25), (24), (4)
Host
Rabbit
(41), (16), (15)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(49), (22), (20), (20), (14), (2), (1), (1), (1), (1)
Pubmed 6 references available
Quantity 400 μL
Options
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Catalog No. ABIN388241
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Immunogen This IDE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-435 AA from the Central region of human IDE.
Clone RB12553
Isotype Ig
Specificity This IDE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413~443 amino acids from the Center region of human IDE.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name IDE
Background IDE belongs to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.
Synonyms: Insulysin, Insulinase, Insulin protease
Molecular Weight 117838 DA
Gene ID 3416
UniProt P14735
Research Area Stem Cells
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Insulin-Degrading Enzyme (IDE) (AA 406-435), (Center) antibody Western blot analysis of anti-IDE Antibody (Center) (ABIN388241) in A375 cell line lysates (35 µg/lane). IDE (arrow) was detected using the purified polyclonal antibody (1:60 dilution).
anti-Insulin-Degrading Enzyme (IDE) (AA 406-435), (Center) antibody (2) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody (Center)(ABIN388241), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Li, Ali, Cohen: "Insulin degrading enzyme is a cellular receptor mediating varicella-zoster virus infection and cell-to-cell spread." in: Cell, Vol. 127, Issue 2, pp. 305-16, 2006 (PubMed).

Radulescu, Hufnagel, Luppa et al.: "Immunohistochemical demonstration of the zinc metalloprotease insulin-degrading enzyme in normal and malignant human breast: correlation with tissue insulin levels." in: International journal of oncology, Vol. 30, Issue 1, pp. 73-80, 2006 (PubMed).

Kim, Hersh, Leissring et al.: "Decreased catalytic activity of the insulin-degrading enzyme in chromosome 10-linked Alzheimer disease families." in: The Journal of biological chemistry, Vol. 282, Issue 11, pp. 7825-32, 2007 (PubMed).

Vepsäläinen, Parkinson, Helisalmi et al.: "Insulin-degrading enzyme is genetically associated with Alzheimer's disease in the Finnish population." in: Journal of medical genetics, Vol. 44, Issue 9, pp. 606-8, 2007 (PubMed).

General Radulescu, Poznic, Pavelic: "Complex formation between metabolic enzymes in tumor cells: unfolding the MDR1-IDE paradigm." in: Molecular cancer therapeutics, Vol. 8, Issue 11, pp. 3171, 2009 (PubMed).

Radulescu, Fischer, Stange et al.: "Promoting scientific standards in Germany." in: Science (New York, N.Y.), Vol. 328, Issue 5976, pp. 307, 2010 (PubMed).

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