E-Ras (N-Term), (AA 13-42) antibody

Details for Product No. ABIN388264
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

 
Antigen
Synonyms HRAS2, HRasp, ecat5, Ha-Ras2, HRASP
Epitope
N-Term, AA 13-42
(7), (6), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(49), (15), (13), (13), (12)
Host
Rabbit
(46), (5)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
(35), (22), (15), (10), (10), (6), (5), (1), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN388264
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Discontinued
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 AA from the N-terminal region of human ERAS.
Clone RB12690
Isotype Ig
Specificity This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20~50 amino acids from the N-terminal region of human ERAS.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name ERAS
Background Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.
Synonyms: E-Ras, Embryonic stem cell-expressed Ras
Molecular Weight 25156 DA
Gene ID 3266
UniProt Q7Z444
Research Area Stem Cells, Cancer, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-E-Ras (N-Term), (AA 13-42) antibody Western blot analysis of ERAS Antibody (N-term) (ABIN388264) in K562 cell line lysates (35 µg/lane). ERAS (arrow) was detected using the purified polyclonal antibody (1:60 dilution).
anti-E-Ras (N-Term), (AA 13-42) antibody (2) Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (N-term) (A28)(ABIN388264), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-E-Ras (N-Term), (AA 13-42) antibody (3) Immunofluorescence analysis of anti-ERAS Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Background publications Miyoshi, Kagimoto, Soeda et al.: "The human c-Ha-ras2 is a processed pseudogene inactivated by numerous base substitutions." in: Nucleic acids research, Vol. 12, Issue 4, pp. 1821-8, 1984 (PubMed).

Takahashi, Mitsui, Yamanaka: "Role of ERas in promoting tumour-like properties in mouse embryonic stem cells." in: Nature, Vol. 423, Issue 6939, pp. 541-5, 2003 (PubMed).

Kameda, Thomson: "Human ERas gene has an upstream premature polyadenylation signal that results in a truncated, noncoding transcript." in: Stem cells (Dayton, Ohio), Vol. 23, Issue 10, pp. 1535-40, 2005 (PubMed).

Hosts (46), (5)
Reactivities (49), (15), (13), (13), (12)
Applications (35), (22), (15), (10), (10), (6), (5), (1), (1), (1), (1)
Conjugates (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (7), (6), (1), (1), (1), (1), (1), (1)
back to top