DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X) (C-Term) antibody

Antigen: DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X)

Synonyms: DBX, DDX3, HLP2, DDX14, Ddx3, Fin14, D1Pas1-rs2, pl10, MGC52935, DDX3X

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388291

Additional Information

Alternative name: DDX3

Gene ID: 1654

UniProt: O00571

Immunogen: This DDX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 463~493 amino acids from the C-terminal region of human DDX3.

Isotype: Ig

Description: Other names: DEAD box protein 3, X-chromosomal, Helicase-like protein 2, HLP2, DEAD box, X isoform, DDX3X, DBX

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DDX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 463~493 amino acids from the C-terminal region of human DDX3.

Molecular Weight: 73113 DA

Comments:

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX3 is a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. The DDX3 gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Stem Cells, Signaling, Chromatin

Restrictions: For Research Use only

Images

Western blot analysis of DDX3 (arrow) using rabbit polyclonal DDX3 Antibody (C-term) (ABIN388291).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDX3 gene (Lane 2) (Origene Technologies).

Western blot analysis of DDX3 Antibody (C-term) (ABIN388291) in Hela cell line lysates (35ug/lane). DDX3 (arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Ariumi, Kuroki, Abe et al.: "DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication." in: Journal of virology, Vol. 81, Issue 24, pp. 13922-6, 2007 (PubMed).