Actin, Alpha, Cardiac Muscle 1 (ACTC1) (Center) antibody

Antigen: Actin, Alpha, Cardiac Muscle 1 (ACTC1)

Synonyms: ACTC, ASD5, CMD1R, CMH11, MGC156490, actcl, actc1l, ACTC1, actc, cmd1r, MGC75679, ACTBL2, MGC128362, ACTA, ASMA, CFTD, MPFD, NEM1, NEM2, NEM3, CFTD1, CFTDM, Acts, Acta-2, Actsk-1, AA959943, fa27h01, fb83f06, wu:fa27h01, wu:fb83f06, ACTA1, acta1, MGC52636, MGC79039, ACTB, acta, asma, cftd, mpfd, nem1, nem2, nem3, acta2, cftd1, cftdm, MGC89192, ACTG1, LOC100154254, DKFZp468G072, Actc-1

Epitope: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388308

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Actin (ACTB/ACTC) (Center)

Gene ID: 70

Swiss-Prot: P68032

Immunogen: This Actin (ACTB/ACTC) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194~224 amino acids from the center region of human ACTB/ACTC.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Actin, cytoplasmic 1, Beta-actin , Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ACTC1

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human ACTB/ACTC.

Molecular Weight: 41737 DA

Synonyms: ACTC, ASD5, CMD1R, CMH11, MGC156490, actcl, actc1l, ACTC1, actc, cmd1r, MGC75679, ACTBL2, MGC128362, ACTA, ASMA, CFTD, MPFD, NEM1, NEM2, NEM3, CFTD1, CFTDM, Acts, Acta-2, Actsk-1, AA959943, fa27h01, fb83f06, wu:fa27h01, wu:fb83f06, ACTA1, acta1, MGC52636, MGC79039, ACTB, acta, asma, cftd, mpfd, nem1, nem2, nem3, acta2, cftd1, cftdm, MGC89192, ACTG1, LOC100154254, DKFZp468G072, Actc-1

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ACTB/ACTC Antibody (Center) in HL60 cell line lysates (35ug/lane). Target protein (arrow) was detected using the purified Pab (1:60 dilution).

Western blot analysis of ACTB/ACTC (arrow) using rabbit polyclonal ACTB/ACTC Antibody (Center) (ABIN388307).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACTB/ACTC gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with ACTB/ACTC antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.