Nucleus Accumbens Associated 1, BEN and BTB (POZ) Domain Containing (NACC1) (C-Term) antibody

Antigen: Nucleus Accumbens Associated 1, BEN and BTB (POZ) Domain Containing (NACC1)

Synonyms: Nac1, Btbd14b, 2010001H03Rik, 4930511N13Rik, NAC1, BEND8, NAC-1, BTBD14B, FLJ37383, Nac-1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388312

Additional Information

Alternative name: Nac1 (BTBD14B)

Gene ID: 112939

UniProt: Q96RE7

Immunogen: This BTBD14B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414~444 amino acids from the C-terminal region of human BTBD14B.

Isotype: Ig

Description: Other names: BTB/POZ domain-containing protein 14B, Nucleus accumbens-1, NAC1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BTBD14B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414~444 amino acids from the C-terminal region of human BTBD14B.

Molecular Weight: 57258 DA

Comments:

Background: BTBD14B functions as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. In humans the protein is upregulated in a number of carcinomas and is localized in discrete nuclear bodies that correlate with tumour recurrence.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50:100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cancer, Transcription Factors, Signaling, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of BTBD14B Antibody (C-term) in mouse heart tissue lysates (35ug/lane). BTBD14B (arrow) was detected using the purified Pab (1:60 dilution).

Publications

Product: Nakayama, Nakayama, Davidson et al.: "A BTB/POZ protein, NAC-1, is related to tumor recurrence and is essential for tumor growth and survival." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 49, pp. 18739-44, 2006 (PubMed).

Davidson, Berner, Trope et al.: "Expression and clinical role of the bric-a-brac tramtrack broad complex/poxvirus and zinc protein NAC-1 in ovarian carcinoma effusions." in: Human pathology, Vol. 38, Issue 7, pp. 1030-6, 2007 (PubMed).

Nakayama, Nakayama, Wang et al.: "NAC-1 controls cell growth and survival by repressing transcription of Gadd45GIP1, a candidate tumor suppressor." in: Cancer research, Vol. 67, Issue 17, pp. 8058-64, 2007 (PubMed).