Cyclin-Dependent Kinase 1 (CDK1) (N-Term), (AA 1-29) antibody

Details for Product No. ABIN388320
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Antigen
Synonyms
5363, CDC2, CDCDm, CDK1, CDK1/CDC2, CG5363, Cdc2, Cdk-1, Cdk1, Dcdc2, Dm cdc2, DmCdc2, DmCdk1, Dmcdc2, Dmel\\CG5363, cdc, cdc2Dm, cdk1, dCdk1, group 4, l(2)31Eh, CDC28A, P34CDC2, Cdc2a, p34, cdc ... show more
5363, CDC2, CDCDm, CDK1, CDK1/CDC2, CG5363, Cdc2, Cdk-1, Cdk1, Dcdc2, Dm cdc2, DmCdc2, DmCdk1, Dmcdc2, Dmel\\CG5363, cdc, cdc2Dm, cdk1, dCdk1, group 4, l(2)31Eh, CDC28A, P34CDC2, Cdc2a, p34, cdc2, wu:fc30e01, zgc:92032, PSTAIR, cdc-2, cdc2-a, cdc28a, cdc2x1.1, p34cdc2, xcdc2, CDC2A, CDC2AAT, CDK2, CDKA1, CDKA;1, CYCLIN-DEPENDENT KINASE A;1, cell division control 2, POL3, pold show less
Epitope
N-Term, AA 1-29
(64), (52), (52), (28), (18), (18), (17), (13), (12), (9), (7), (7), (6), (6), (6), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(478), (249), (236), (45), (37), (30), (27), (27), (16), (7), (6), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(394), (124)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(21), (15), (15), (9), (9), (9), (6), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS)
(430), (198), (115), (110), (64), (63), (60), (46), (26), (21), (10), (9), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN388320
291.50 $
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Immunogen This CDC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 AA from the N-terminal region of human CDC2.
Clone RB13695
Isotype Ig
Specificity This CDC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8~37 amino acids from the N-terminal region of human CDC2.
Predicted Reactivity Cow (Bovine),Chicken
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name CDC2
Background CDC2 is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
Synonyms: Cell division control protein 2 homolog, p34 protein kinase, Cyclin-dependent kinase 1, CDK1
Molecular Weight 34095 DA
Gene ID 983
UniProt P06493
Research Area Apoptosis/Necrosis, Stem Cells, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Cyclin-Dependent Kinase 1 (CDK1) (N-Term), (AA 1-29) antibody Western blot analysis of CDC2 Antibody (N-term) in 293 cell line lysates (35 µg/lane). CDC2 (arrow) was detected using the purified polyclonal antibody (1:60 dilution).
anti-Cyclin-Dependent Kinase 1 (CDK1) (N-Term), (AA 1-29) antibody (2) Western blot analysis of CDC2 (arrow) using rabbit polyclonal CDC2 Antibody (N-term) (ABIN388320).293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the CDC2 gene (Lane 2) (Origene Technologies).
anti-Cyclin-Dependent Kinase 1 (CDK1) (N-Term), (AA 1-29) antibody (3) Flow cytometric analysis of 293 cells using CDC2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Skoufias, Indorato, Lacroix et al.: "Mitosis persists in the absence of Cdk1 activity when proteolysis or protein phosphatase activity is suppressed." in: The Journal of cell biology, Vol. 179, Issue 4, pp. 671-85, 2007 (PubMed).

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