Cell-Division-Cycle Kinase 2 (cdc2) (C-Term) antibody

Antigen: Cell-Division-Cycle Kinase 2 (cdc2)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388321

Additional Information

Alternative name: CDC2

Gene ID: 983

UniProt: P06493

Immunogen: This CDC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220~249 amino acids from the C-terminal region of human CDC2.

Isotype: Ig

Description: Other names: Cell division control protein 2 homolog, p34 protein kinase, Cyclin-dependent kinase 1, CDK1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CDC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220~249 amino acids from the C-terminal region of human CDC2.

Molecular Weight: 34095 DA

Comments:

Background: CDC2 is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of CDC2 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Stem Cells, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CDC2 (arrow) using rabbit polyclonal CDC2 Antibody (C-term) (ABIN388321).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDC2 gene (Lane 2) (Origene Technologies).

Publications

Product: Skoufias, Indorato, Lacroix et al.: "Mitosis persists in the absence of Cdk1 activity when proteolysis or protein phosphatase activity is suppressed." in: The Journal of cell biology, Vol. 179, Issue 4, pp. 671-85, 2007 (PubMed).