Toll-Like Receptor 9 (TLR9) (C-Term) antibody

Antigen: Toll-Like Receptor 9 (TLR9)

Synonyms: CD289

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388351

Additional Information

Alternative name: TLR9

Gene ID: 81897

UniProt: Q9EQU3

Immunogen: This Mouse TLR9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 963~992 amino acids from the C-terminal region of mouse TLR9.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Mouse TLR9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 963~992 amino acids from the C-terminal region of mouse TLR9.

Molecular Weight: 116347 DA

Comments:

Background: TLR9 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR9 is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

The anti-TLR9 Pab (ABIN388351) is used in Western blot to detect TLR9 in mouse spleen tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

TLR9 Antibody (R881) (ABIN388351) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the TLR9 antibody detected the TLR9 protein (arrow).

Publications

Product: Hemmi, Takeuchi, Kawai et al.: "A Toll-like receptor recognizes bacterial DNA." in: Nature, Vol. 408, Issue 6813, pp. 740-5, 2000 (PubMed).

Bauer, Kirschning, Häcker et al.: "Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 16, pp. 9237-42, 2001 (PubMed).

Chuang, Lee, Kline et al.: "Toll-like receptor 9 mediates CpG-DNA signaling." in: Journal of leukocyte biology, Vol. 71, Issue 3, pp. 538-44, 2002 (PubMed).