Signal Transducer and Activator of Transcription 3 (STAT3) (S727) antibody

Antigen: Signal Transducer and Activator of Transcription 3 (STAT3)

Synonyms: APRF, HIES, FLJ20882, MGC16063, Aprf, AW109958, 1110034C02Rik, MGC93551, MGC128731, STAT3, stat3

Epitope: S727

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388367

Additional Information

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Phospho-STAT3-S727

Gene ID: 6774

Swiss-Prot: P40763

Immunogen: This Phospho-STAT3-S727 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT3.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Signal transducer and activator of transcription 3, Acute-phase response factor, APRF

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT3.

Molecular Weight: 88068 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

The phospho-STAT3-Ser727 Pab (ABIN388367) is used in Western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before (-) or after (+) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).

The phospho-STAT3-Ser727 Pab (ABIN388367) is used in Western blot to detect Ser727-phosphorylated STAT3 in HL-60 tissue lysates collected before (-) or after (+) stimulation obtained by IFN-a.

Confocal immunofluorescent analysis of Phospho-STAT3-S727 Antibody(ABIN388367) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).