Gap Junction Protein, alpha 1, 43kd (GJA1) (N-Term) antibody

Antigen: Gap Junction Protein, alpha 1, 43kd (GJA1)

Synonyms: CX43, GJAL, ODDD, DFNB38, Cx43, Npm1, Cnx43, Gja-1, AU042049, AW546267, Cx43alpha1, connexin43, Cx32, Cnx32, Gjb-1, MGC6217, AI118175, MGC93610, gja1, cx43a1, zfCx43.3, MGC128721, gja1-A, connexin 43, cx43, gjal, oddd, dfnb38, CONNEXIN 43

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388369

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Connexin 43 (GJA1) (N-term)

Gene ID: 2697

Swiss-Prot: P17302

Immunogen: This GJA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114~144 amino acids from the N-terminal region of human GJA1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Gap junction alpha-1 protein, Connexin 43, Cx43, Gap junction 43 kDa heart protein

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human GJA1.

Molecular Weight: 42877 DA

Synonyms: CX43, GJAL, ODDD, DFNB38, Cx43, Npm1, Cnx43, Gja-1, AU042049, AW546267, Cx43alpha1, connexin43, Cx32, Cnx32, Gjb-1, MGC6217, AI118175, MGC93610, gja1, cx43a1, zfCx43.3, MGC128721, gja1-A, connexin 43, cx43, gjal, oddd, dfnb38, CONNEXIN 43

Application Details

Application Notes: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

(LEFT)Western blot analysis of anti-GJA1 Antibody (N-term)(ABIN388369) in CEM cell line lysates (35ug/lane). GJA1(arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of hGJA1-N121.Connexin (ABIN388369) in mouse brain tissue lysates (35ug/lane).GJA1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Publications: Li, Zhang, Jiao et al.: "Knockdown of microRNA-181 by lentivirus mediated siRNA expression vector decreases the arrhythmogenic effect of skeletal myoblast transplantation in rat with myocardial infarction." in: Microvascular research, Vol. 78, Issue 3, pp. 393-404, 2009 (PubMed).