Gap Junction Protein, delta 2, 36kDa (GJD2) (C-Term) antibody

Antigen: Gap Junction Protein, delta 2, 36kDa (GJD2)

Synonyms: CX36, GJA9, MGC138315, MGC138319, Cx35.1, GJD2, Cx36, GJA10

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388383

Additional Information

Alternative name: Connexin 36 / GJA9

Gene ID: 57369

UniProt: Q9UKL4

Immunogen: This Connexin 36 / GJA9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292~321 amino acids from the C-terminal region of human GJA9.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Connexin 36 Antibody antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292~321 amino acids from the C-terminal region of human GJA9.

Molecular Weight: 36092 DA

Comments:

Background: GJA9, also called connexin-36 (CX36), is a member of the connexin gene family that is expressed predominantly in mammalian neurons. Connexins associate in groups of 6 and are organized radially around a central pore to form connexons. Each gap junction intercellular channel is formed by the conjunction of 2 connexons.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology, Cell Structure

Restrictions: For Research Use only

Images

The anti-GJA9 C-term Pab (ABIN388383) is used in Western blot to detect GJA9 in placenta tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: de Brouwer, Pennings, Roeters et al.: "Mutations in the calcium-binding motifs of CDH23 and the 35delG mutation in GJB2 cause hearing loss in one family." in: Human genetics, Vol. 112, Issue 2, pp. 156-63, 2003 (PubMed).