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Finkel-Biskis-Reilly Murine Sarcoma Virus (FBR-MuSV) Ubiquitously Expressed (FAU) (C-Term) antibody
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This FAU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101~130 amino acids from the C-terminal region of human FUBI.|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This FAU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101~130 amino acids from the C-terminal region of human FUBI.|
|Molecular Weight||14630 DA|
Background: This gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50-100 Immunoprecipitation ~~ Not tested Flow cytometry ~~ Not tested|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Ubiquitin-related antibodies, Translation Factors, Protein Modifications, Signaling|
|Restrictions||For Research Use only|
Michiels, Van der Rauwelaert, Van Hasselt et al.: "fau cDNA encodes a ubiquitin-like-S30 fusion protein and is expressed as an antisense sequence in the Finkel-Biskis-Reilly murine sarcoma virus." in: Oncogene, Vol. 8, Issue 9, pp. 2537-46, 1993 (PubMed).
Vladimirov, Ivanov, Karpova et al.: "Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry." in: European journal of biochemistry / FEBS, Vol. 239, Issue 1, pp. 144-9, 1996 (PubMed).
Kenmochi, Kawaguchi, Rozen et al.: "A map of 75 human ribosomal protein genes." in: Genome research, Vol. 8, Issue 5, pp. 509-23, 1998 (PubMed).
|Hosts||Rabbit (16), Mouse (1)|
|Reactivities||Human (16), Mouse (Murine) (5), Rat (Rattus) (4), Cow (Bovine) (2), Dog (Canine) (2), Cat (Feline) (1), Chicken (1), Pig (Porcine) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (17), ELISA (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (IHC) (3), Immunofluorescence (IF) (1), Immunoprecipitation (IP) (1)|
|Epitopes||N-Term (3), C-Term (2), Center (1), Internal Region (1)|