Finkel-Biskis-Reilly Murine Sarcoma Virus (FBR-MuSV) Ubiquitously Expressed (FAU) (C-Term) antibody

Antigen: Finkel-Biskis-Reilly Murine Sarcoma Virus (FBR-MuSV) Ubiquitously Expressed (FAU)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388406

Additional Information

Alternative name: FAU

Gene ID: 2197

UniProt: NP_001988

Immunogen: This FAU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101~130 amino acids from the C-terminal region of human FUBI.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This FAU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101~130 amino acids from the C-terminal region of human FUBI.

Molecular Weight: 14630 DA

Comments:

Background: This gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50-100 Immunoprecipitation ~~ Not tested Flow cytometry ~~ Not tested

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Translation Factors, Protein Modifications, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of anti-FUBI C-term Pab (ABIN388406) in Hela and T47D cell line lysates. FUBI(arrow) was detected using the purified Pab.

Western blot analysis of anti-hFUBI C-term Pab (ABIN388406) in 293 cell line lysates transiently transfected with the FAU gene (2ug/lane). FUBI(arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with FUBI Antibody (C-term)(ABIN388406), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Michiels, Van der Rauwelaert, Van Hasselt et al.: "fau cDNA encodes a ubiquitin-like-S30 fusion protein and is expressed as an antisense sequence in the Finkel-Biskis-Reilly murine sarcoma virus." in: Oncogene, Vol. 8, Issue 9, pp. 2537-46, 1993 (PubMed).

Vladimirov, Ivanov, Karpova et al.: "Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry." in: European journal of biochemistry / FEBS, Vol. 239, Issue 1, pp. 144-9, 1996 (PubMed).

Kenmochi, Kawaguchi, Rozen et al.: "A map of 75 human ribosomal protein genes." in: Genome research, Vol. 8, Issue 5, pp. 509-23, 1998 (PubMed).