|+1 404 474 4654|
|+1 888 205 9894 (TF)|
LC3 (APG8A) (Isoform B) antibody
Alternatives Western Blotting (WB), ELISA
|5 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the Center region of human MAP1LC3A (Isoform b specific).|
|Description||Other names: MAP1LC3B, LC3B, MAP1A/1BLC3, MAP1LC3B|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This LC3 Antibody antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the Center region of human MAP1LC3A (Isoform b specific).|
|Molecular Weight||14272 DA|
Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Signaling, Cell Structure, Autophagy|
|Restrictions||For Research Use only|
|Western blot analysis of Autophagy APG8a (MAP1LC3A) antibody (Isoform b specific) (ABIN388472) in Y79 cell line lysates (35ug/lane). MAP1LC3A(arrow) was detected using the purified Pab.|
He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).
Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).
Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).
Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).
Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).
|Reactivities||Human (9), Rat (Rattus) (1)|
|Applications||ELISA (9), Western Blotting (WB) (9), Immunohistochemistry (IHC) (3), Immunofluorescence (IF) (2), Immunocytochemistry (ICC) (1)|
|Epitopes||Thr93, Tyr99 (2), Asp106 (1), Ile31 (1), N-Term (1), Pro45 (1), Ser12 (1), Ser3 (1)|