Did you know that you can buy products from over 140 different suppliers from us?

LC3 (APG8B) (N-Term), (AA 1-30) antibody

Details for Product No. ABIN388473
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Epitope
N-Term, AA 1-30
(1), (1)
Reactivity
Human, Rat (Rattus)
(1), (1)
Host
Rabbit
(1)
Clonality (Clone)
Polyclonal ()
Application
Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)
(1)
Pubmed 8 references available
Quantity 400 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN388473
238.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human LC3.
Clone RB7481
Isotype Ig
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background Function:
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Cellular Location:
Cytoplasm, cytoskeleton. Endomembrane system, Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane, Lipid-anchor. Note=LC3-II binds to the autophagic membranes Localizes also to discrete punctae along the ciliary axoneme (By similarity)

Tissue Location:
Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver

Synonyms: MAP1LC3B, LC3B, MAP1A/1BLC3, MAP1LC3B
Molecular Weight 14557 Da
Gene ID 81631
UniProt Q9GZQ8
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes Suggested dilutions:
IF = 1:100, WB = 1:1000, IHC = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1.2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-LC3 (APG8B) (N-Term), (AA 1-30) antibody Western blot analysis of anti-LC3 (APG8b) Pab (ABIN388473) in rat brain lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in membrane fraction (P) but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).
anti-LC3 (APG8B) (N-Term), (AA 1-30) antibody (2) Western blot analysis of anti-LC3 (APG8b) Pab (ABIN388473) in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2 µg/lane). Human LC3 (APG8b) (arrow) was detected using the purified polyclonal antibody (1:60 dilution).
anti-LC3 (APG8B) (N-Term), (AA 1-30) antibody (3) FACS sorted LSK cells (Lineage-Sca-1+c-kit+, enriched for hematopoietic stem cells) incubated with LC3 antibody (ABIN388473) at 1:50 dilution at 4 degree overnight., and detected with AlexaFlour 488 conjugated anti-rabbit antibody (1:200). Data courtesy of Wenbin Xiao, La Jolla Institute for Allergy and Immunology.
Product cited in: Ramírez, Pham, Franco et al.: "Hydroquinone induces oxidative and mitochondrial damage to human retinal Müller cells (MIO-M1)." in: Neurotoxicology, Vol. 39C, pp. 102-108, 2013 (PubMed).

Background publications He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

General English, Chemali, Duron et al.: "Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection." in: Nature immunology, Vol. 10, Issue 5, pp. 480-7, 2009 (PubMed).

Viiri, Hyttinen, Ryhaenen et al.: "p62/sequestosome 1 as a regulator of proteasome inhibitor-induced autophagy in human retinal pigment epithelial cells." in: Molecular vision, Vol. 16, pp. 1399-414, 2010 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN388473
238.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
Did you look for something else?
back to top