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Details for Product No. ABIN388473

LC3 (APG8B) (N-Term), (AA 1-30) antibody

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Antigen
Epitope
»Alternatives N-Term, AA 1-30
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality (Clone) Polyclonal ()
Application
»Alternatives Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 8 references available
Catalog no. ABIN388473
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human LC3.
Clone RB7481
Isotype Ig
Specificity This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human LC3 (APG8b).
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Synonyms: MAP1LC3B, LC3B, MAP1A/1BLC3, MAP1LC3B
Molecular Weight 14557 Da
Gene ID 81631
UniProt Q9GZQ8
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes Recommend dilutions: IF : 1:100,WB : 1:1000,IHC : 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 1.2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Product cited in: Ramírez, Pham, Franco et al.: "Hydroquinone induces oxidative and mitochondrial damage to human retinal Müller cells (MIO-M1)." in: Neurotoxicology, Vol. 39C, pp. 102-108, 2013 (PubMed).

Background publications He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

English, Chemali, Duron et al.: "Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection." in: Nature immunology, Vol. 10, Issue 5, pp. 480-7, 2009 (PubMed).

Viiri, Hyttinen, Ryhaenen et al.: "p62/sequestosome 1 as a regulator of proteasome inhibitor-induced autophagy in human retinal pigment epithelial cells." in: Molecular vision, Vol. 16, pp. 1399-414, 2010 (PubMed).

Alternatives for antigen "LC3 (APG8B)", type "Antibodies"
Hosts (2)
Reactivities (2), (1)
Applications (2), (1)
Epitopes (1), (1), (1), (1)
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