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APG8b (MAP1LC3B) (Center), (Thr93), (Tyr99) antibody

Details for Product No. ABIN388476, Supplier: Log in to see
Antigen
Epitope
Center, Thr93, Tyr99
1
1
1
1
1
Reactivity
Human
3
1
Host
Rabbit
3
Clonality
Polyclonal
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
2
1
1
1
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Immunogen This APG8b (MAP1LC3B)-T93/Y99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-111 amino acids from the central region of human PMAT-Y198.
Specificity This APG8b (MAP1LC3B)-T93/Y99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-111 amino acids from the central region of human PMAT-Y198.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Synonyms: MAP1LC3B,MAP1ALC3
Molecular Weight 14688 DA
Gene ID 81631
NCBI Accession NP_073729
UniProt Q9GZQ8
Research Area Cell Structure, Autophagy
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Comment

Background: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/ml
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-APG8b (MAP1LC3B) (Center), (Thr93), (Tyr99) antibody (ABIN388476) Western blot analysis of APG8b (MAP1LC3B) Antibody (T93/Y99) (ABIN388476) in MCF-7 ce...
Western Blotting (WB) image for anti-APG8b (MAP1LC3B) (Center), (Thr93), (Tyr99) antibody (ABIN388476) APG8b (MAP1LC3B)-T93/Y99 (AP1802f) western blot analysis in mouse cerebellum tissue l...
Immunohistochemistry (IHC) image for anti-APG8b (MAP1LC3B) (Center), (Thr93), (Tyr99) antibody (ABIN388476) APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) (ABIN388476) immunohistochemistry analysis...
Background publications Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome. ..." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).

Tanida, Ueno, Kominami: "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 47704-10, 2004 (PubMed).

He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Tanida-Miyake, Nishitani et al.: "Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs." in: Biochemical and biophysical research communications, Vol. 292, Issue 1, pp. 256-62, 2002 (PubMed).

Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).

Kabeya, Mizushima, Ueno et al.: "LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing." in: The EMBO journal, Vol. 19, Issue 21, pp. 5720-8, 2000 (PubMed).