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LC3 (APG8A/B) (AA 23-52) antibody

Details for Product No. ABIN388477, Supplier: Log in to see
Antigen
Epitope
AA 23-52
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Host
Rabbit
Clonality (Clone)
Polyclonal ()
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Immunogen This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 AA from human LC3.
Clone RB7483-RB7484
Isotype Ig
Specificity This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34~64 amino acids surrounding Y38 of human LC3 (APG8a/b).
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a and MAP1LC3b are light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecules are cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Synonyms: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate
Molecular Weight 14272 & 14557 DA
Gene ID 81631
UniProt Q9GZQ8
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-LC3 (APG8A/B) (AA 23-52) antibody (ABIN388477) Western blot analysis of anti-Autophagy LC3 Antibody (APG8a/b) (ABIN388477) in Y79 ce...
Western Blotting (WB) image for anti-LC3 (APG8A/B) (AA 23-52) antibody (ABIN388477) Western blot analysis of anti-Autophagy LC3 Antibody (APG8a/b) (ABIN388477) in mouse ...
Immunohistochemistry (IHC) image for anti-LC3 (APG8A/B) (AA 23-52) antibody (ABIN388477) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).