MAP1LC3A antibody (N-Term)

Catalog No. ABIN388478

Product Details anti-MAP1LC3A Antibody

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Binding Specificity: AA 1-30, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.

Clone: RB7613

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-MAP1LC3A antibody (ABIN388478)

Liu, He, von Rütte, Yousefi, Hunger, Simon: "Down-regulation of autophagy-related protein 5 (ATG5) contributes to the pathogenesis of early-stage cutaneous melanoma." in: Science translational medicine, Vol. 5, Issue 202, pp. 202ra123, (2013) (PubMed).

Thyagarajan, Jedinak, Nguyen, Terry, Baldridge, Jiang, Sliva: "Triterpenes from Ganoderma Lucidum induce autophagy in colon cancer through the inhibition of p38 mitogen-activated kinase (p38 MAPK)." in: Nutrition and cancer, Vol. 62, Issue 5, pp. 630-40, (2010) (PubMed).

Choi, Kim, Ha, Kim, Son: "A novel mTOR activating protein protects dopamine neurons against oxidative stress by repressing autophagy related cell death." in: Journal of neurochemistry, Vol. 112, Issue 2, pp. 366-76, (2010) (PubMed).

Liao, Cheung, Galeano, Ji, Qin, Bi: "Allopregnanolone treatment delays cholesterol accumulation and reduces autophagic/lysosomal dysfunction and inflammation in Npc1-/- mouse brain." in: Brain research, Vol. 1270, pp. 140-51, (2009) (PubMed).

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: LC3 (MAP1LC3A Products)

Synonyms: ATG8E antibody, LC3 antibody, LC3A antibody, MAP1ALC3 antibody, MAP1BLC3 antibody, 1010001H21Rik antibody, 4922501H04Rik antibody, LC3a antibody, MGC69006 antibody, zgc:77094 antibody, MGC89867 antibody, MAP1LC3A antibody, map1lc3a antibody, microtubule associated protein 1 light chain 3 alpha antibody, microtubule-associated protein 1 light chain 3 alpha antibody, microtubule associated protein 1 light chain 3 alpha S homeolog antibody, MAP1LC3A antibody, Map1lc3a antibody, map1lc3a.S antibody, map1lc3a antibody

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3c is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Molecular Weight: 16852

Gene ID: 440738

NCBI Accession: NP_001004343

UniProt: Q9BXW4

Pathways: Autophagy