LC3 (APG8C) (AA 27-57), (Thr48) antibody

Details for Product No. ABIN388481
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Antigen
Epitope
AA 27-57, Thr48
(1), (1)
Reactivity
Human
(1), (1), (1)
Host
Rabbit
(1)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB)
(1), (1)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN388481
291.50 $
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Immunogen This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-57 AA from human LC3.
Clone RB17599
Isotype Ig
Specificity This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35~64 amino acids surrounding T48 of human MAP1LC3C.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3c is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Synonyms: MAP1LC3C, microtubule-associated protein 1 light chain 3 gamma, LC3C, MAP1LC3C
Molecular Weight 16852 DA
Gene ID 440738
UniProt Q9BXW4
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.317 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-LC3 (APG8C) (AA 27-57), (Thr48) antibody Western blot analysis of APG8c (MAP1LC3C) Antibody (T48)(ABIN388481) in Hela cell line lysates (35 µg/lane). MAP1LC3C (arrow) was detected using the purified polyclonal antibody.
Background publications He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

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