ATG3 Autophagy Related 3 Homolog (S. Cerevisiae) (ATG3) antibody

Antigen: ATG3 Autophagy Related 3 Homolog (S. Cerevisiae) (ATG3)

Synonyms: APG3, APG3L, PC3-96, FLJ22125, MGC15201, APG3-LIKE, DKFZp564M1178, Apg3l, Atg3l, 2610016C12Rik, Pig1, PIG-1, atg3, zgc:64156, wu:fb15g12

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388490

Additional Information

Alternative name: ATG3

Gene ID: 64422

UniProt: Q9NT62

Immunogen: This ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~33 amino acids surrounding Y18 of human APG3L.

Isotype: Ig

Description: Other names: APG3, APG3-LIKE, APG3L, DKFZp564M1178, FLJ22125, MGC15201, PC3-96, 2610016C12Rik, APG3 autophagy 3-like, autophagy Apg3p/Aut1p-like, autophagy-related protein 3, hApg3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~33 amino acids surrounding Y18 of human APG3L.

Molecular Weight: 35864 DA

Comments:

Background: APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8(MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blot~~1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Autophagy

Restrictions: For Research Use only

Images

Western blot analysis of Phospho-APG3L-pY18 (ABIN388490) in T47D cell line lysates (35ug/lane). APG3L (arrow) was detected using the purified Pab.

Western blot analysis of Phospho-APG3L-pY18 (ABIN388490) in mouse lung tissue lysates (35ug/lane). APG3L (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APG3L Antibody (Y18) (ABIN388490), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).