|+1 404 474 4654|
|+1 888 205 9894 (TF)|
ATG3 Autophagy Related 3 Homolog (S. Cerevisiae) (ATG3) antibody
|Synonyms||APG3, APG3L, PC3-96, FLJ22125, MGC15201, APG3-LIKE, DKFZp564M1178, Apg3l, Atg3l, 2610016C12Rik, Pig1, PIG-1, atg3, zgc:64156, wu:fb15g12|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|5 references available|
|Quantity||400 µL (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~33 amino acids surrounding Y18 of human APG3L.|
|Description||Other names: APG3, APG3-LIKE, APG3L, DKFZp564M1178, FLJ22125, MGC15201, PC3-96, 2610016C12Rik, APG3 autophagy 3-like, autophagy Apg3p/Aut1p-like, autophagy-related protein 3, hApg3|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~33 amino acids surrounding Y18 of human APG3L.|
|Molecular Weight||35864 DA|
Background: APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8(MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blot~~1:50~100 Immunohistochemistry ~~ 1:10~50|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Signaling, Cell Structure, Autophagy|
|Restrictions||For Research Use only|
Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).
Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).
Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).
Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).
Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).