ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae) (ATG4A) (AA 1-30) antibody

Details for Product No. ABIN388491
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Antigen
Synonyms APG4A, AUTL2, AI627006, AV169859, Apg4a, Atg4al, Autl2, Aut2a, zgc:111958, apg4a, autl2, F6E13.27
Epitope
AA 1-30
(12), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(53), (25), (16), (13), (1), (1)
Host
Rabbit
(53), (1), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(28), (20), (17), (16), (10), (7), (2), (2), (1)
Pubmed 5 references available
Catalog no. ABIN388491
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This ATG4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from human ATG4A.
Clone RB7493
Isotype Ig
Specificity This ATG4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7~37 amino acids from the N-terminal region of human APG4A.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ATG4A
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.
Synonyms: RP5-889N15.2, APG4A, AUTL2, APG4 autophagy 4 homolog A, AUT-like 2 cysteine endopeptidase, AUT-like 2, cysteine endopeptidase, autophagin 2, autophagin-2, autophagy-related cysteine endopeptidase 2, autophagy-related protein 4 homolog A, cysteine protease
Molecular Weight 45378 DA
Gene ID 115201
UniProt Q8WYN0
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.45 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae) (ATG4A) (AA 1-30) antibody The anti-APG4A Pab (ABIN388491) is used in Western blot to detect APG4A in mouse kidney (left) and mouse heart (right) tissue lysates
anti-ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae) (ATG4A) (AA 1-30) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

Hosts (53), (1), (1)
Reactivities (53), (25), (16), (13), (1), (1)
Applications (28), (20), (17), (16), (10), (7), (2), (2), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (12), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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