ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae) (ATG4B) antibody

Antigen: ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae) (ATG4B)

Synonyms: Apg4b, MGC112887, ATG4B, APG4B, Aut2b2, apg4b, Atg4b, AUTL1, MGC1353

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388495

Additional Information

Alternative name: ATG4B

Gene ID: 23192

UniProt: Q9Y4P1

Immunogen: This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85~115 amino acids surrounding amino acid G93 of human APG4B.

Isotype: Ig

Description: Other names: APG4B, AUTL1, MGC1353, APG4 autophagy 4 homolog B, AUT-like 1 cysteine endopeptidase, autophagin-1, autophagy-related cysteine endopeptidase 1, autophagy-related protein 4 homolog B, cysteine protease ATG4B, hAPG4B

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85~115 amino acids surrounding amino acid G93 of human APG4B.

Molecular Weight: 44309 DA

Comments:

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Autophagy

Restrictions: For Research Use only

Images

Western blot analysis of anti-APG4B Pab (ABIN388495) in mouse brain tissue lysate. APG4B(arrow) was detected using the purified Pab.

Western blot analysis of anti-hAPG4B-G93 Pab (ABIN388495) in 293 cell line lysates transiently transfected with the ATG4B gene (2ug/lane). hAPG4B-G93(arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).