ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae) (ATG4B) antibody

Antigen: ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae) (ATG4B)

Synonyms: Apg4b, MGC112887, ATG4B, APG4B, Aut2b2, apg4b, Atg4b, AUTL1, MGC1353

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388498

Additional Information

Alternative name: ATG4B

Gene ID: 23192

UniProt: Q9Y4P1

Immunogen: This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126~155 amino acids from the Center region of human APG4B.

Isotype: Ig

Description: Other names: APG4B, AUTL1, MGC1353, APG4 autophagy 4 homolog B, AUT-like 1 cysteine endopeptidase, autophagin-1, autophagy-related cysteine endopeptidase 1, autophagy-related protein 4 homolog B, cysteine protease ATG4B, hAPG4B

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126~155 amino acids from the Center region of human APG4B.

Molecular Weight: 44309 DA

Comments:

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:50~100 Immunohistochemistry ~~ 1:50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Autophagy

Restrictions: For Research Use only

Images

Western blot analysis of anti-Autophagy APG4B Antibody (E135) (ABIN388498) in HL60 cell line lysates (35ug/lane). APG4B(arrow) was detected using the purified Pab (1:60 dilution).

Western blot analysis of APG4B (arrow) using rabbit polyclonal APG4B Antibody (E135) (ABIN388498).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APG4B gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy APG4B antibody (E135)(ABIN388498), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).