ATG4 Autophagy Related 4 Homolog C (S. Cerevisiae) (ATG4C) (N-Term), (AA 33-62) antibody

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Antigen
Synonyms apg4c, atg4cl, zgc:86888, wu:fc56g07, APG4-C, APG4C, AUTL1, AUTL3, Apg4-C, Apg4c, Atg4cl, Autl1
Epitope
N-Term, AA 33-62
(12), (12), (12), (12), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(91), (80), (67), (48), (48), (48), (1)
Host
Rabbit
(96), (12), (3), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(61), (50), (25), (22), (17), (12), (4), (3), (2), (1)
Pubmed 5 references available
Quantity 400 µL
Options
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Catalog No. ABIN388503
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Immunogen This ATG4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 AA from the N-terminal region of human ATG4C.
Clone RB7561
Isotype Ig
Specificity This ATG4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40~70 amino acids from the N-term of human APG4C.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ATG4C
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Synonyms: APG4-C, APG4C, AUTL1, AUTL3, FLJ14867, APG4 autophagy 4 homolog C, AUT-like 1, cysteine endopeptidase, AUT-like 3 cysteine endopeptidase, autophagin-3, autophagy-related cysteine endopeptidase 3, autophagy-related protein 4 homolog C, cysteine protease AT
Molecular Weight 52497 DA
Gene ID 84938
UniProt Q96DT6
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-ATG4 Autophagy Related 4 Homolog C (S. Cerevisiae) (ATG4C) (N-Term), (AA 33-62) antibody Western blot analysis of anti-hAPG4C-Y48 Pab (ABIN388503) in 293 cell line lysates transiently transfected with the ATG4C gene (2 µg/lane). hAPG4C-Y48(arrow) was detected using the purified polyclonal antibody (1:60 dilution).
anti-ATG4 Autophagy Related 4 Homolog C (S. Cerevisiae) (ATG4C) (N-Term), (AA 33-62) antibody (2) The anti-APG4C Pab (ABIN388503) is used in Western blot to detect APG4C in mouse liver tissue lysate
anti-ATG4 Autophagy Related 4 Homolog C (S. Cerevisiae) (ATG4C) (N-Term), (AA 33-62) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

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