ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (N-Term), (AA 22-51) antibody

Details for Product No. ABIN388520
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Antigen
Epitope
N-Term, AA 22-51
(13), (12), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(59), (25), (15), (1), (1)
Host
Rabbit
(55), (3), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(44), (19), (14), (12), (12), (10), (8), (3), (3), (1), (1), (1)
Pubmed 7 references available
Quantity 400 µL
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Catalog No. ABIN388520
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Immunogen This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-51 AA from the N-terminal region of human ATG7.
Clone RB7467
Isotype Ig
Specificity This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29~59 amino acids from the N-term of human APG7L.
Predicted Reactivity Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ATG7
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.
Synonyms: APG5, APG5-LIKE, APG5L, ASP, hAPG5, APG5 (autophagy 5, S. cerevisiae)-like, APG5 autophagy 5-like, ATG5 autophagy related 5-like, OTTHUMP00000017824, apoptosis specific protein, apoptosis-specific protein, autophagy protein 5
Molecular Weight 77960 DA
Gene ID 10533
UniProt O95352
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (N-Term), (AA 22-51) antibody Western blot analysis of anti-Autophagy APG7L Antibody (N-term) (ABIN388520) in 293 cell line lysates transiently transfected with the ATG7 gene (3 µg/lane). APG7L (arrow) was detected using the purified polyclonal antibody.
anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (N-Term), (AA 22-51) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (N-Term), (AA 22-51) antibody (3) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy APG7L Antibody (N-term) (ABIN388520), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).

Tanida, Tanida-Miyake, Nishitani et al.: "Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs." in: Biochemical and biophysical research communications, Vol. 292, Issue 1, pp. 256-62, 2002 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

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