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ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 540-569), (C-Term) antibody

Details for Product No. ABIN388523, Supplier: Login to see
Antigen
  • AO090001000744
  • atg7
  • APG7-LIKE
  • APG7L
  • GSA7
  • 1810013K23Rik
  • Agp7
  • Apg7l
  • Atg7l
  • ATAPG7
  • ATATG7
  • ATG7
  • AUTOPHAGY 7
  • AUTOPHAGY-RELATED 7
  • K15I22.10
  • K15I22_10
Epitope
AA 540-569, C-Term
29
15
15
14
13
8
7
5
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
144
40
24
Host
Rabbit
138
10
3
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
4
4
4
4
4
4
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
115
61
55
32
21
17
10
7
6
3
3
2
1
1
Supplier
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Immunogen This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 AA from the C-terminal region of human ATG7.
Clone RB7474
Isotype Ig
Specificity This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 547~577 amino acids from the C-term of human APG7L.
Predicted Reactivity Chicken,Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ATG7 (ATG7 Antibody Abstract)
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.
Synonyms: APG7-LIKE, APG7L, DKFZp434N0735, GSA7, APG7 autophagy 7-like, autophagy-related protein 7, hAGP7, ubiquitin activating enzyme E1-like protein, ubiquitin-activating enzyme E1-like protein
Pathways: Regulation of autophagy
Molecular Weight 77960 Da
Gene ID 10533
UniProt O95352
Research Area Cell Signaling, Cell Structure, Autophagy
Pathways
Application Notes IF = 1:25, WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 540-569), (C-Term) antibody (ABIN388523) Western blot analysis of APG7L (C-term) Pab (ABIN388523) in 293 cell line lysates tra...
Western Blotting (WB) image for anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 540-569), (C-Term) antibody (ABIN388523) Western blot analysis of APG7L Pab (ABIN388523) in 293 cell line lysate (35 µg/lane)....
Immunohistochemistry (IHC) image for anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 540-569), (C-Term) antibody (ABIN388523) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autoph...
Product cited in: Ding, Shi, Zhou et al.: "Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma." in: Cancer research, Vol. 68, Issue 22, pp. 9167-75, 2008 (PubMed).

Background publications Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Tanida, Tanida-Miyake, Nishitani et al.: "Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs." in: Biochemical and biophysical research communications, Vol. 292, Issue 1, pp. 256-62, 2002 (PubMed).

Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).