Images for product: anti-GABA(A) Receptor-Associated Protein (GABARAP) (AA 1-30), (N-Term) antibody
Western blot analysis of GABARAP (arrow) using purified polyclonal antibody (ABIN388564). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (Lane 2) (Origene Technologies).
Western blot done using standard conditions on WT MEFs, starved with EBSS for 2 hours with and without leupeptin before lysing. Primary antibodies used were ABIN388564 Rabbit anti-GABARAP, 1:250 in 5% milk (in PBS + 0.1% Tween-20) and rabbit anti-actin (Abcam). Secondary detection by anti-rabbit-HRP (Sigma), ECL exposure 2 minutes( provided by Fiona McAlpine & Sharon Tooze).