DNA-Damage Regulated Autophagy Modulator 2 (DRAM2) (C-Term) antibody

Antigen: DNA-Damage Regulated Autophagy Modulator 2 (DRAM2)

Synonyms: DRAM, FLJ11259, Dram, 1200002N14Rik, PRO180, TMEM77, WWFQ154, MGC54289, RP5-1180E21.1, fj35e01, zgc:86754, wu:fj35e01, dram, DRAM1, flj11259, MGC108229, MGC187403, DRAM2, MGC128340

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388570

Additional Information

Alternative name: DRAM -2

Gene ID: 55332

UniProt: Q8N682

Immunogen: This DRAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DRAM.

Isotype: Ig

Description: Other names: Damage-regulated autophagy modulator

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DRAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DRAM.

Molecular Weight: 26122 DA

Comments:

Background: DRAM is regulated as part of the p53 tumor suppressor pathway. It is a lysosomal membrane protein that is required for the induction of autophagy by the pathway. Decreased transcriptional expression of the gene encoding DRAM is associated with various tumors.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Cancer, Autophagy, Transcription Factors, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of anti-DRAM (C-term)-2 Pab (ABIN388570) in NCI-H460 cell line lysates (35ug/lane). DRAM (arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DRAM (C-term)-2, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Green, Chipuk: "p53 and metabolism: Inside the TIGAR." in: Cell, Vol. 126, Issue 1, pp. 30-2, 2006 (PubMed).

Kerley-Hamilton, Pike, Hutchinson et al.: "The direct p53 target gene, FLJ11259/DRAM, is a member of a novel family of transmembrane proteins." in: Biochimica et biophysica acta, Vol. 1769, Issue 4, pp. 209-19, 2007 (PubMed).