UV Radiation Resistance Associated Gene (UVRAG) (C-Term,Leu555) antibody

Antigen: UV Radiation Resistance Associated Gene (UVRAG)

Synonyms: p63, DHTX, Uvrag1, Uvragl, AI648770, BB124205, 9530039D02Rik, MGC56164, zgc:56164, UVRAG, uvrag, MGC81810, MGC131135

Binding Site: C-Term,Leu555

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388589

Additional Information

Alternative name: UVRAG

Gene ID: 7405

UniProt: Q9P2Y5

Immunogen: This UVRAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 547~577 amino acids from the C-terminal region of human UVRAG.

Isotype: Ig

Description: Other names: UV radiation resistance-associated gene protein, p63

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This UVRAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 547~577 amino acids from the C-terminal region of human UVRAG.

Molecular Weight: 78151 DA

Comments:

Background: UVRAG complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving the UVAG gene are associated with left-right axis malformation and mutations in the gene have been associated with colon cancer.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Autophagy, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of UVRAG Antibody (C-term L555) (ABIN388589) in Ramos cell line lysates (35ug/lane). UVRAG (arrow) was detected using the purified Pab (1:60 dilution).

Publications

Product: Perelman, Dafni, Naiman et al.: "Molecular cloning of a novel human gene encoding a 63-kDa protein and its sublocalization within the 11q13 locus." in: Genomics, Vol. 41, Issue 3, pp. 397-405, 1997 (PubMed).

Ionov, Nowak, Perucho et al.: "Manipulation of nonsense mediated decay identifies gene mutations in colon cancer Cells with microsatellite instability." in: Oncogene, Vol. 23, Issue 3, pp. 639-45, 2004 (PubMed).

Liang, Feng, Ku et al.: "Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG." in: Nature cell biology, Vol. 8, Issue 7, pp. 688-99, 2006 (PubMed).