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PI3KC3 (C-Term), (AA 770-801) antibody

Details for Product No. ABIN388591
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Antigen
Epitope
C-Term, AA 770-801
(4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(15), (1), (1), (1), (1), (1)
Host
Rabbit
(15)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(9), (8), (6), (4), (1)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN388591
291.50 $
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Immunogen This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 770-801 AA from the C-terminal region of human PI3KC3.
Clone RB10857
Specificity This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263~292 amino acids from the C-terminal region of human PI3KC3.
Predicted Reactivity Mouse (Murine),Pig (Porcine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.
Synonyms: Phosphatidylinositol 3-kinase catalytic subunit type 3, PtdIns-3-kinase type 3, PI3-kinase type 3, PI3K type 3, Phosphoinositide-3-kinase class 3, Phosphatidylinositol 3-kinase p100 subunit, vps34, PI 3 Kinase Class 3
Molecular Weight 101549 DA
Gene ID 5289
UniProt Q8NEB9
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Autophagy
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-PI3KC3 (C-Term), (AA 770-801) antibody Western blot analysis of PI3KC3 Antibody (C-term E785)(ABIN388591) against transiently transfected HEK-293 cell line lysate (1 µg/lane). PI3KC3 (arrow) was detected using the purified polyclonal antibody (1:1500 dilution).
anti-PI3KC3 (C-Term), (AA 770-801) antibody (2) Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with hPI3KC3 (C-term) (ABIN388591), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Volinia, Dhand, Vanhaesebroeck et al.: "A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system." in: The EMBO journal, Vol. 14, Issue 14, pp. 3339-48, 1995 (PubMed).

Vergne, Chua, Deretic: "Tuberculosis toxin blocking phagosome maturation inhibits a novel Ca2+/calmodulin-PI3K hVPS34 cascade." in: The Journal of experimental medicine, Vol. 198, Issue 4, pp. 653-9, 2003 (PubMed).

Validation Images
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