YEATS Domain Containing 4 (N-Term,Met1) antibody

Antigen: YEATS Domain Containing 4

Synonyms: RGD1305741, MGC68689, zgc:100841, MGC89432, YEATS4, YAF9, GAS41, NUBI-1, 4930573H17Rik, B230215M10Rik, NuBI-1, MGC133571, dGas41, DmelCG9207, CG9207, GB13136, GLIOMAS 41, K2N11.8, K2N11_8, TAF14B, TBP-ASSOCIATED FACTOR 14B, NV13456, Gas41

Binding Site: N-Term,Met1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388605

Additional Information

Alternative name: GAS41 (YEATS4/NuBI-1)

Gene ID: 8089

UniProt: O95619

Immunogen: This GAS41 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human NuBI-1.

Isotype: Ig

Description: Other names: YEATS domain-containing protein 4, Glioma-amplified sequence-41, Gas41, NuMA binding protein 1, NuBI-1, NuBI1, YEATS4, GAS41,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GAS41 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human NuBI-1.

Molecular Weight: 26499 DA

Comments:

Background: NuBI-1 is found in the nucleoli. It has high sequence homology to human MLLT1, and yeast and human MLLT3 proteins. Both MLLT1 and MLLT3 proteins belong to a class of transcription factors, indicating that the encoded protein might also represent a transcription factor. This protein is thought to be required for RNA transcription. The gene for this protein has been shown to be amplified in tumors.

Synonyms: RGD1305741, MGC68689, zgc:100841, MGC89432, YEATS4, YAF9, GAS41, NUBI-1, 4930573H17Rik, B230215M10Rik, NuBI-1, MGC133571, dGas41, DmelCG9207, CG9207, GB13136, GLIOMAS 41, K2N11.8, K2N11_8, TAF14B, TBP-ASSOCIATED FACTOR 14B, NV13456, Gas41

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of NuBI-1 (arrow) using rabbit polyclonal NuBI-1 Antibody (N-term M1)(ABIN388605). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YEATS4 gene (Lane 2) (Origene Technologies).

Publications

Product: Munnia, Schütz, Romeike et al.: "Expression, cellular distribution and protein binding of the glioma amplified sequence (GAS41), a highly conserved putative transcription factor." in: Oncogene, Vol. 20, Issue 35, pp. 4853-63, 2001 (PubMed).

Debernardi, Bassini, Jones et al.: "The MLL fusion partner AF10 binds GAS41, a protein that interacts with the human SWI/SNF complex." in: Blood, Vol. 99, Issue 1, pp. 275-81, 2001 (PubMed).

Zimmermann, Ahrens, Matthes et al.: "Targeted disruption of the GAS41 gene encoding a putative transcription factor indicates that GAS41 is essential for cell viability." in: The Journal of biological chemistry, Vol. 277, Issue 21, pp. 18626-31, 2002 (PubMed).

Lauffart, Gangisetty, Still: "Molecular cloning, genomic structure and interactions of the putative breast tumor suppressor TACC2." in: Genomics, Vol. 81, Issue 2, pp. 192-201, 2003 (PubMed).

Cai, Jin, Tomomori-Sato et al.: "Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex." in: The Journal of biological chemistry, Vol. 278, Issue 44, pp. 42733-6, 2003 (PubMed).