UPF1 Regulator of Nonsense Transcripts Homolog (Yeast) (UPF1) (N-Term) antibody

Antigen: UPF1 Regulator of Nonsense Transcripts Homolog (Yeast) (UPF1)

Synonyms: HUPF1, NORF1, RENT1, pNORF1, FLJ43809, FLJ46894, KIAA0221, Rent1, Upflp, PNORF-1, B430202H16Rik, rENT1, rent1, zgc:55472, wu:fi40f07, wu:fj48a01, UPF1, Tb05.3C6.50

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388607

Additional Information

Alternative name: RENT1

Gene ID: 5976

UniProt: Q92900

Immunogen: This RENT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304~334 amino acids from the N-terminal region of human RENT1.

Isotype: Ig

Description: Other names: Regulator of nonsense transcripts 1, ATP-dependent helicase RENT1, Nonsense mRNA reducing factor 1, NORF1, Up-frameshift suppressor 1 homolog, hUpf1, RENT1, KIAA0221, UPF1,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RENT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304~334 amino acids from the N-terminal region of human RENT1.

Molecular Weight: 124345 DA

Comments:

Background: RENT1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western Blot ~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Cell Signaling, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of RENT1 (arrow) using rabbit polyclonal RENT1 Antibody (N-term) (ABIN388607). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RENT1 gene (Lane 2) (Origene Technologies).

Publications

Product: Mendell, ap Rhys, Dietz: "Separable roles for rent1/hUpf1 in altered splicing and decay of nonsense transcripts." in: Science (New York, N.Y.), Vol. 298, Issue 5592, pp. 419-22, 2002 (PubMed).

Ohnishi, Yamashita, Kashima et al.: "Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7." in: Molecular cell, Vol. 12, Issue 5, pp. 1187-200, 2003 (PubMed).